A-259 Detecting Human RNase P Gene Prevents False-Negative of SARS-CoV-2

Abstract Background Real-time Reverse transcriptase—polymerase chain reaction (RT-PCR) is a regular technique for detecting SARS-CoV-2 infection. Human RNase P sequence using as internal control PCR assay usually. This article discusses the correlation between result and human gene result. Methods Nasopharyngeal swabs were collected from two patients within three consecutive days. We detected target by real-time RT-PCR assay, which includes E gene, Rarp N . And threshold cycle values (Ct) determined cobas® z 480. Evaluate results of detection. All samples also analysis infection in Taiwan Centers Disease Control. Results consistent both laboratories. genes specimens on day1. Ct 29.63 29.50. Rdrp 32.23 32.50. 33.09 32.68. 21.21 21.22. On day 2 3, all negative PCR. 25.73 26.16 29.82 30.28 3. Conclusion The detection can be correlated with numbers cells. Comparing days, value related to Collection factor affect Develop acceptance range quality standard sample collection prevent false-negative SARS-CoV-2.